Paint pen set for Yamaha DRM2 deep red 20 ml + clear varnish 20 ml

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Discussion

A) In vitro methylation assay of DRM2 or C397R on DNA with different sequence contexts. CG, (GAC) 12; CTA, (TAC) 12; CAA, (AAC) 12; CTG, (TGC) 12. Data are means ± SD ( n = 3 replicates). Statistical analysis used two-tailed Student’s t test for the difference from WT: * P< 0.05, *** P< 0.001, and **** P< 0.0001. ( B) Ribbon representation of DRM2 C397R bound to CCG DNA and SAH. Hydrogen-bonding interactions formed between the side chain of R397 and G12 are depicted as dashed lines in expanded view. The bases of C11 and G12 in the expanded view are colored yellow. ( C) Metaplots showing average methylation level of DRM2, C397R, and ddc over TEs for CG, CHG, and CHH contexts. ( D) Representative genomic regions of two TEs on chromosome 3 (AT3TE666360 and AT3TE28430/AT3TE28440) showing the methylation levels of Col-0, ddc, DRM2/ ddc, and C397R/ ddc. ( E) Bar chart showing the total number of DMCs in each context of DRM2/ ddc and C397R/ ddc called against ddc. ( F) Motif of the 4 nucleotides upstream and 5 nucleotides downstream of hyper DMCs in C397R called against ddc ( n = 29,347). Johnson LM, Law JA, Khattar A, Henderson IR, Jacobsen SE (2008) SRA-domain proteins required for DRM2-mediated de novo DNA methylation. PLoS Genet 4: e1000280. Smith LM, Pontes O, Searle I, Yelina N, Yousafzai FK, et al. (2007) An SNF2 protein associated with nuclear RNA silencing and the spread of a silencing signal between cells in Arabidopsis. Plant Cell 19: 1507–1521. For crystallization, the DRM2-DNA complexes were each mixed with 1 mM SAH. Crystals for all the DRM2-DNA complexes were generated using sitting-drop vapor-diffusion method at 4°C. Each drop was prepared by mixing 0.5 μl of DRM2-DNA complex sample with 0.5 μl of precipitant solution [for DRM2-CTT, DRM2-CCG, and DRM2 C397R-CCG complexes: 2% v/v Tacsimate (pH 6.0), 0.1 M bis-tris (pH 6.5), and 20% w/v polyethylene glycol 3350; for DRM2-CAT complex: 0.1 M sodium acetate trihydrate (pH 7.0) and 12% w/v polyethylene glycol 3350; for DRM2-CCT complex: 0.1 M sodium formate (pH 7.0) and 12% w/v polyethylene glycol 3350; and for DRM2-CTG: 0.2 M potassium iodide and 20% w/v polyethylene glycol 3350 (pH 7.0)]. The crystal quality was further improved using the microseeding method. To harvest crystals, the crystals were soaked in cryoprotectants made of mother liquor supplemented with 30% glycerol before being flash-frozen in liquid nitrogen.