Cats: 1

Product Information

Gordon D, Huddleston J, Chaisson MJ, Hill CM, Kronenberg ZN, Munson KM, et al. Long-read sequence assembly of the gorilla genome. Science. 2016;352(6281):aae0344. Epub 2016/04/02. pmid:27034376; PubMed Central PMCID: PMC4920363. D'Alessandro, Anthony (8 November 2018). " 'Cats' Cast: Royal Ballet Principal Dancer Steven McRae To Play Skimbleshanks". Archived from the original on 8 November 2018 . Retrieved 8 November 2018. Masso, Giverny (16 April 2019). "Naoimh Morgan: 'Acting in the Cats movie is a dream come true' | Interviews". Archived from the original on 16 April 2019 . Retrieved 16 April 2019. Lang, Brent (3 December 2013). " 'Cats' Movie May Happen, Lord Andrew Lloyd Webber Says". TheWrap. Archived from the original on 20 July 2018 . Retrieved 21 February 2019.

Lewis, Rebecca (18 December 2019). "And because it's what everyone wants to know, Taylor Swift is one of the few genuinely good parts of the film although she doesn't appear until the very end. Unsure why they cut Bombularina from early parts of the film unless they wanted (needed?) that anticipation. #CatsMoviepic". @bexlewis361. Archived from the original on 19 December 2019 . Retrieved 19 December 2019. In the veterinary clinic, the practice of genomic medicine is impending [ 1]. With actionable genetic information in hand, companion animal therapeutic interventions are feasible, including treatment of animal patients prior to, or to prevent the appearance of, more severe symptoms and allow therapeutic administration of drugs with higher efficacy and fewer side effects. Genomic information can also alert veterinarians to imminent disease risks for diagnostic consideration. Each of these applications could significantly enhance veterinary medicine, however, none are currently in practice. As in human medicine, formidable challenges exist for the implementation of genomic-based medicine, including accurate annotation of the genome and databases of genetic variation from well-phenotyped individuals that include both single nucleotide variant (SNV) and structural variant (SV) discovery and annotation [ 2– 4]. Targeted individual companion animal genome information is becoming more readily available, cost effective, and tentatively linked to the actionable phenotypes via direct-to-consumer DNA testing. Thus correct interpretation of DNA variants is of the utmost importance for communicating findings to clinicians practicing companion animal genomic medicine [ 5].Mahamdallie S, Yost S, Poyastro-Pearson E, Holt E, Zachariou A, Seal S, et al. Identification of new Wilms tumour predisposition genes: an exome sequencing study. Lancet Child Adolesc Health. 2019;3(5):322–31. Epub 2019/03/20. pmid:30885698; PubMed Central PMCID: PMC6472290. Vigne J-D, Guilaine J, Debue K, Haye L, Gérard P. Early taming of the cat in Cyprus. Science. 2004;304(5668):259-. pmid:15073370 Gudbjartsson DF, Helgason H, Gudjonsson SA, Zink F, Oddson A, Gylfason A, et al. Large-scale whole-genome sequencing of the Icelandic population. Nat Genet. 2015;47(5):435–44. Epub 2015/03/26. pmid:25807286.

Lang, Brent (6 October 2021). "Andrew Lloyd Webber on Broadway's Reopening, 'Cinderella' and Why the 'Cats' Movie Caused Him to Buy a Dog". Variety . Retrieved 7 October 2021. Hamlet, Isaac (18 February 2020). "Why one of the Midwest's most cutting-edge theaters is screening 2019's biggest box-office bomb". Iowa City Press-Citizen . Retrieved 26 February 2020.

Methods

Lam ET, Hastie A, Lin C, Ehrlich D, Das SK, Austin MD, et al. Genome mapping on nanochannel arrays for structural variation analysis and sequence assembly. Nature biotechnology. 2012;30(8):771. pmid:22797562 To the left of the figure panels, constraint groups are labeled with pLI ranges shown below in braces. Below the pLI ranges are the number of genes found in each constraint group. SNV values show the total number of SNVs of a particular impact that belong to each constraint group. (a) Observed percentage differences from expected values for per kb SNV density. Light colored rectangles represent the 95% confidence intervals for the expected values. Confidence intervals were calculated using 10,000 permutations (methods). (b) The percentage of SNVs of different impacts and constraint groups across various MAF intervals. (c) Observed percentage differences from expected values for the fraction of SNVs in a singleton state (allele count of 1). Light colored rectangles represent the 95% confidence intervals for the expected values, generated from 10,000 permutations. (d) Pairwise SNV count contrasts between gene constraint groups and SNVs of the same impact. “*” indicates a p-value < 0.05 and “**” indicates a p-value < 0.001. (e) Pairwise singleton count contrasts between gene constraint groups and SNVs of the same impact. Asterisks above odds ratio values indicate the same values as (d). Dewey FE, Murray MF, Overton JD, Habegger L, Leader JB, Fetterolf SN, et al. Distribution and clinical impact of functional variants in 50,726 whole-exome sequences from the DiscovEHR study. Science. 2016;354(6319):aaf6814. pmid:28008009 Victoria, a young white cat, is abandoned by her owner in the streets of London in the middle of the night. The alley cats, witnessing this introduce themselves to her as the " Jellicles". Mr. Mistoffelees, Munkustrap, Cassandra and Demeter take Victoria under their wing and show her the world of the Jellicles as they prepare for the Jellicle Ball, an annual ceremony where cats must compete to be chosen to go to the Heaviside Layer and be granted a new life.

Cats (2019)". Metacritic. Fandom, Inc. Archived from the original on 25 December 2019 . Retrieved 19 February 2021. Louwerens M, London CA, Pedersen NC, Lyons LA. Feline lymphoma in the post-feline leukemia virus era. J Vet Intern Med. 2005;19(3):329–35. Epub 2005/06/16. pmid:15954547. D'Alessandro, Anthony (18 October 2018). "Judi Dench Set To Pounce On 'Cats' ". Deadline Hollywood. Archived from the original on 19 October 2018 . Retrieved 18 October 2018.

Author summary

DePristo MA, Banks E, Poplin R, Garimella KV, Maguire JR, Hartl C, et al. A framework for variation discovery and genotyping using next-generation DNA sequencing data. Nature genetics. 2011;43(5):491. pmid:21478889

Improved sensitivity for smaller SV events, while helpful for finding new disease causing mutations, may also lead to increased false positive SV detection. In three human trios sequenced with Illumina technology, LUMPY and Delly were both used to identify 12,067 and 5,307 SVs respectively, contributing to a unified call set, along with several other tools, of 10,884 SVs per human on average [ 77]. In cats, the average number of SVs per individual was 4 times higher than in humans, with 44,990 SVs per cat, suggesting the total number of SVs in cats is likely inflated. However, the majority of the SVs were at population frequencies below 0.5, ruling out poor reference assembly as a contributing factor. Instead, the difference in SV count between cats and humans is likely due to sample specific factors. For example, the majority of samples were sequenced using two separate libraries of 350 bp and 550 bp insert sizes ( S2 Data). Despite the potentially high number of false positive SVs, increased SV sensitivity was useful for trait discovery. The deletion associated with dwarfism was only found in the Delly2 call set. If SV filtering were more stringent, such as requiring SVs to be called by both callers, the feline dwarfism SV may not have otherwise been detected. Ultimately, these results highlight the importance of high sensitivity for initial SV discovery and the use of highly specific molecular techniques for downstream validation of candidate causative SVs. Fire the Cat Cannon to blast baddies getting too close to your base! Overcome weird enemies with the right Cat squad and take down the enemy base! Spycher M, Bauer A, Jagannathan V, Frizzi M, De Lucia M, Leeb T. A frameshift variant in the COL5A1 gene in a cat with Ehlers-Danlos syndrome. Anim Genet. 2018;49(6):641–4. Epub 2018/09/25. pmid:30246406.

Cats': Taylor Swift, Idris Elba prowl the red carpet for New York premiere". USA Today. 16 December 2019. Archived from the original on 17 December 2019 . Retrieved 17 December 2019.