Southwire 58282240 MCCUT BX/MC Rotary Cable Cutter with Lever

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Dutta K, Dasu VV. Synthesis of short chain alkyl esters using cutinase from Burkholderia cepacia NRRL B2320. J Mol Catal B Enzym. 2011;72:150–6.

Born on May 23, 1987, Ronald "Ronnie" McNutt was an Army Reserve veteran who lived in the US city of New Albany, Mississippi, where he worked at a Toyota plant, was involved in the local theatre scene and co-hosted a podcast called JustUs Geeks.Coldewey, Devin (September 13, 2020). "Graphic video of suicide spreads from Facebook to TikTok to YouTube as platforms fail moderation test". techcrunch.com. TechCrunch . Retrieved March 24, 2022. Cutinases have attracted great interest in recent years due to their great application potential in different fields, including biodiesel production, ester synthesis, textile, and detergent as well as environmental industries [ 9, 31]. So far, many cutinases have been cloned and expressed to meet industrial requirements for large-scale production [ 8, 13, 14, 15, 16]. However, expression levels of cutinases are generally low. Here we first cloned a novel cutinase gene ( McCut) from a thermophilic fungus M. cinnamomea and successfully over-expressed in P. pastoris. Since the yeast is adaptable for large-scale fermentation for the production of recombinant proteins, high-cell density fermentation was carried out in a 5-L fermentor. The cutinase activity reached a maximum of 12, 536 U/mL with a protein concentration of 10.8 g/L. Many cutinases from Alternaria brassicicola [ 32], A. niger [ 33], G. cingulata [ 15], and Sirococcus conigenus [ 34] have been expressed in P. pastoris. Among those, the production yield of G. cingulata cutinase is the highest, with a protein concentration of 3.8 g/L and an enzyme activity of 434 U/mL [ 15], which are much lower than those observed in this study. Some cutinases expressed in other hosts were also performed in bioreactor systems for scale-up production [ 8, 13, 14, 16]. The cutinase gene from T. fusca was expressed in E. coli with a yield of 5.1 g/L cutinase (2, 258.5 U/mL) in a 3-L fermentor, which is the highest activity level ever reported [ 16]. Overall, the production level reported in this study represents the highest activity for a cutinase to date, making McCut a potential cutinase for industrial-scale production. In 2003, Martine landed the role as tea-lady in Lovely Actually, where the British Prime Minister, played by Hugh Grant, falls in love with her character. The star claimed she'd only wanted time out to launch her pop career but was written out the script as punishment - and found out while listening to the radio. Reynolds, Nigel (3 November 2001). "McCutcheon quits show over fears for her voice". Telegraph. London, UK. Archived from the original on 30 August 2006 . Retrieved 15 April 2012.

Mr McNutt shared a tragic final Facebook post before he began the broadcast. It stated: "Someone in your life needs to hear that they matter. That they are loved. The synthetic reactions by McCut were carried out in 5 mL isooctane containing 0.1 M butyric acid, 0.2 M butanol, 200–600 U/mL of spray-dried cutinase powder, and 40 mg/mL molecular sieve 4 Å. The mixtures were incubated at 40–70 °C with constant shaking at 200 rpm. Otwinowski Z, Minor W. Processing of X-ray diffraction data collected in oscillation mode, macromolecular crystallography. Methods Enzymol. 1997;276:307–26.The specific primers McCutF (5′-ATGAAGATCCAATTTGTTATTTCCGC-3′) and McCutR (5′-TTACGAGAGTCTATCCTCAAGCC-3′) were designed. To obtain the full-length gene and coding sequence, PCR was performed using genomic DNA and cDNA as templates, respectively. PCR conditions were as follows: a hot start at 94 °C for 5 min, 35 cycles of 94 °C for 30 s, 54 °C for 30 s, and 72 °C for 60 s, followed by a final extension step at 72 °C for 10 min. After amplification, PCR products were purified, ligated to pMD18-T vector, and sequenced. The younger Ronnie is survived by his mum Elaine, brother Joey, sister Mindy, three nieces and two nephews, according to an obituary.

a b c Cortés, Michelle Santiago. "Let's Talk About The Graphic Video Going Viral On TikTok". www.refinery29.com. Refinery29 . Retrieved March 24, 2022.

How do I report distressing videos on TikTok?

However, Mal Young, the BBC's controller of drama series at the time, admitted they 'fell out' but said the show refused to be her 'safety net'. Two days after his suicide, McNutt was buried in Snowdown Church of Christ Cemetery in Prentiss County, Mississippi. [11] Viral spread [ edit ] Qin Z, Yan Q, Yang S, Jiang Z. Modulating the function of a β-1,3-glucanosyltransferase to that of an endo-β-1,3-glucanase by structure-based protein engineering. Appl Microbiol Biotechnol. 2016;100:1765–76. The Masked Singer UK's Swan unmasked as Martine McCutcheon leaving fans stunned". Daily Mirror. 9 January 2021.

Video platform TikTok was also slow to respond to the video, which had appeared in many user feeds and constant re-uploads, leading to many users choosing to boycott the platform. [1] The case has raised awareness about both suicide prevention and what duty of care social media platforms owe to protect users and victims' reputations online when it comes to graphic content. [2] [3] Life and career [ edit ] The footage, which was originally shared on Facebook is being reshared on various social media platforms including Tik Tok. A piece of my mind: Martine McCutcheon". The Herald. 16 November 2009. Archived from the original on 17 March 2014 . Retrieved 24 November 2009. Dutta K, Sen S, Veeranki VD. Production, characterization and application of microbial cutinases. Process Biochem. 2009;44:127–34.

Have friends and family spoken?

a b "EastEnders cleans up". BBC News. 3 September 1998. Archived from the original on 22 June 2015 . Retrieved 28 March 2009. Xu H, Yan Q, Duan X, Yang S, Jiang Z. Characterization of an acidic cold-adapted cutinase from Thielavia terrestris and its application in flavor ester synthesis. Food Chem. 2015;188:439–45. For isolation of genomic DNA, M. cinnamomea S168 was cultured at 37 °C for 3 days with a rotation speed of 200 rpm. Fungal mycelia were collected by centrifugation at 11, 510 g for 10 min, then washed twice with sterilized water, and ground to powder in liquid nitrogen. The genomic DNA was extracted using a Fungal DNA Midi Kit (Omega Biotek, Doraville, GA, USA). The total RNA was isolated with Trizol reagent (Invitrogen, Carlsbad, USA) and mRNAs were purified using the Oligotex mRNA Midi kit (Qiagen, Germany). First and second strands of cDNA were synthesized using PrimeScript™ RT-PCR Kit (TaKaRa, Tokyo, Japan). The New Albany Police Department had been called to the scene during the livestream, but did not enter McNutt's apartment until after they heard the fatal gunshot, his phone was still ringing as officers searched the scene. Police Chief Chris Robertson reported that his officers had secured the perimeter and evacuated nearby residents before attempting to communicate with McNutt via speakerphone, to no avail. [9] The case sparked a debate over what legal liability is owed by internet platforms that fail to promptly remove graphic and disturbing footage from public view, with the blame generally being placed on Facebook for failing to cut off the livestream during the initial suicide attempt itself. Joshua Steen had called Facebook multiple times, and had called the police, neither of which stopped the stream before McNutt had already committed suicide. [23] [24] Steen declared, "if some woman posts a topless photo, their software will detect that, remove it, and ban their account. That's apparently more offensive than my friend killing himself." [25] It was argued by the two platforms that the " dark web" was responsible for the ongoing circulation of the video. [26] [27]