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Roland Cy-5 Dual Trigger Cymbal Pad, 10In

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For protein labeling, Cy3 and Cy5 dyes sometimes bear a succinimidyl group to react with amines, or a maleimide group to react with a sulfhydryl group of cysteine residues. While patent protection for the standard Cy series of dyes has lapsed, the trademarked Cy naming remains in place. Consequently, dyes that are identical to Cy dyes, but called different names, are now sold. a b Ernst LA, Gupta RK, Mujumdar RB, Waggoner AS (Jan 1989). "Cyanine dye labeling reagents for sulfhydryl groups". Cytometry. 10 (1): 3–10. doi: 10.1002/cyto.990100103. PMID 2917472. Ranasinghe, R. T. & Brown, T. Ultrasensitive fluorescence-based methods for nucleic acid detection: Towards amplification-free genetic analysis. Chem. Commun. 47, 3717–3735 (2011).

Among currently available fluorescent dyes, the cyanine dyes are better able to withstand the harsh dehydration and embedding conditions required for mounting sections in non-polar plastic media, such as DPX and Permount™. The cyanine dyes are brighter in the non-polar environment than in an aqueous medium, resulting in less acquisition time in the confocal microscope than that required for DyLight and Alexa Fluor® dyes (Figure 1), even though those dyes are brighter in aqueous mounting media. Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. CytoCy5S: The use of this product in an NTR gene reporter assay is the subject of US patent number 7,579,140 in the name of Cytiva. The pH value of protein solution shall be 8.5±0.5. If the pH is lower than 8.0, 1 M sodium bicarbonate shall be used for adjustment. Doktycz, M. J., Paner, T. M., Amaratunga, M. & Benight, A. S. Thermodynamic stability of the 5’ dangling-ended DNA hairpins formed from sequences 5’-(Xy)2ggatac(T)4gtatcc-3’, Where X, Y = a, T, G, C. Biopolymers 30, 829–845 (1990).Cy3B: This product is manufactured under an exclusive license from Carnegie Mellon University and is covered by US patent number 6,133,445 and equivalent patents and patent applications in other countries. Cy3 fluoresces greenish yellow (~550 nm excitation, ~570nm emission), while Cy5 is fluorescent in the far-red region (~650 excitation, 670nm emission). [12] Cy3 can be detected by various fluorometers, imagers, and microscopes with standard filters for Tetramethylrhodamine (TRITC). Due to its high molar extinction coefficient, this dye is also easily detected by naked eye on electrophoresis gels, and in solution.

Levitus, M. & Ranjit, S. Cyanine dyes in biophysical research: the photophysics of polymethine fluorescent dyes in biomolecular environments. Q Rev. Biophys. 44, 123–151 (2011).

General properties

a b Mujumdar B, Ernst A, Mujumdar SR, Lewis CJ, Waggoner AS (Mar 1993). "Cyanine dye labeling reagents: Sulfoindocyanine succinimidyl esters". Bioconjugate Chemistry. 4 (2): 105–111. doi: 10.1021/bc00020a001. PMID 7873641. Mujumdar, R. B., Ernst, L. A., Mujumdar, S. R., Lewis, C. J. & Waggoner, A. S. Cyanine dye labeling reagents—sulfoindocyanine Succinimidyl Esters. Bioconjugate Chem. 4, 105–111 (1993).

Nie, Q.; Li, C.; Wang, Y.; Hu, Y.; Pu, W.; Zhang, Q.; Cai, J.; Lin, Y.; Li, G.; Wang, C.; Li, L.; Dou, Y.; Zhang, J. Pathologically Triggered in Situ Aggregation of Nanoparticles for Inflammation-Targeting Amplification and Therapeutic Potentiation. Acta Pharmaceutica Sinica B, 2023, 13(1), 390–409. doi: 10.1016/j.apsb.2022.07.013 The design of DNA oligonucleotides is based on a permutation scheme allowing for all possible combinations of 5 consecutive nucleotides immediately adjacent to a fluorescent dye to be synthesized in parallel as microarrays (P 1–P 5 in Fig. 1). The total amount of unique pentanucleotides is 4 5, or 1024 oligonucleotides to be synthesized. To account for potential variability in synthesis efficiency which would produce lower fluorescence signals for poorly-synthesized sequences, the nucleotide content in all DNA sequences is kept constant by adding a subsection composed of five “N” trinucleotides (N 1 to N 5) where each N x corresponds to AGCT minus the nucleotide in P x. Between the P and N sections, a T 15 spacer is introduced and the entire oligonucleotide sequence is then synthesized over a T 5 linker separating the DNA from the surface of the array. At the 3′ end of the oligonucleotide, immediately after the pentanucleotide, a Cy3 or Cy5 dye is attached. Schematically, all 1024 combinations are represented in the form 3′Cy3/Cy5—P 1P 2P 3P 4P 5—T 15—(ACGT-P 1)—(ACGT-P 2)—(ACGT-P 3)—(ACGT-P 4)—(ACGT-P 5)—T 5—glass. Microarray synthesisIris Dyes | Cyanine Technologies". Archived from the original on 2015-01-26 . Retrieved 2015-01-26. CyQ (or Cy5Q or Cy7Q): These products are covered under US patent number 6,828,116 and equivalent patents and patent applications in other countries in the name of Cytiva. Many analogs of standard Cy 2 / 3 / 3.5 / 5 / 5.5 / 7 / 7.5 dyes were developed, using diverse modification: The protein must be in the buffer without primary amine (such as Tris or glycine) and ammonium ion, otherwise the labeling efficiency will be affected. requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available

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